Genetic Engineering: DNA Technology and Gene Expression Control - Lecture 4

Explore advanced DNA technology concepts in this 52-minute lecture from Yale University's Frontiers of Biomedical Engineering course. Delve into RNA silencing methods, including anti-sense therapy and RNA interference, for controlling gene expression. Learn about molecular cloning techniques for protein mass-production using plasmids, restriction enzymes, ligase, and antibiotic selection in bacteria. Discover the steps and molecules involved in polymerase chain reaction (PCR). Understand how to detect mutations in genomic DNA through gel electrophoresis and Southern blotting, with a focus on sickle cell anemia. Examine a practical application of genetic engineering by studying the induction and control of insulin expression in large animals using the insulin gene and lac promoter. The lecture covers six key chapters: Introduction, Mechanisms of Limiting Gene Expression, Plasmids, Restriction Enzymes, Polymerase Chain Reaction, and Applications of PCR.