Learn the fundamental principles and mechanisms of molecular biology through a comprehensive course covering DNA replication, RNA transcription, protein translation, gene expression regulation, signal transduction pathways, and recombinant DNA technology. Master the intricacies of DNA polymerase, RNA polymerase, genetic codes, and protein synthesis systems while exploring both prokaryotic and eukaryotic cellular processes. Examine key concepts like the Lac operon, λ repressor, chromatin remodeling, and various signaling pathways including cAMP-PKA and EGF. Gain practical knowledge of recombinant DNA techniques, including tool enzymes, vector selection, gene cloning, and transformation methods. Develop a strong foundation in molecular biology concepts essential for understanding modern biotechnology and genetic engineering applications.
Overview
Syllabus
- Chaper 1 DNA Replication
- 1. Fundamental Rules of DNA Replication
- 2. DNA Polymerase
- 3. Enzymes and protein factors required in DNA replication
- 4. DNA replication in prokaryotes
- 5. DNA replication in eukaryotes
- 6. Reverse Transcription
- Chapter 2 RNA Transcription
- 1. DNA Template and Promoter in Transcription
- 2. RNA Polymerase
- 3. Prokaryotic transcription
- 4. Eukaryotic transcription
- 5. RNA Processing
- Chapter 3 Protein Translation
- 1. Genetic codon
- 2. System of protein synthesis
- 3. Activation of amino acids
- 4. Initiation of Protein Synthesis
- 5. Elongation of Protein Synthesis
- 6. Termination of Protein Synthesis
- 7. Posttranslational processing
- Chapter 4 The Regulation of Gene Expression
- 1. Introduction and Prokaryotic DNA-Binding Proteins
- 2. Lac operon
- 3. The λ repressor
- 4. Tryptophan operon
- 5. Eukaryotic Cis-acting elements
- 6. Eukaryotic Transcription Factors
- 7. Chromatin Remodeling
- Chapter 5 Signal Transduction Pathways
- 1. Introduction AND Primary messenger
- 2. Second Messengers
- 3. G protein
- 4. Receptors
- 5. cAMP-PKA pathway
- 6. Enzyme Coupled Receptor Insulin signal pathway
- 7. EGF signal pathway
- Chapter 6 Recombinant DNA Technology
- 1. Recombinant DNA Technology & Common Tool Enzyme
- 2. Target Gene Acquisition
- 3. Vector & Vector Selection
- 4. Formation & Transformation of Recombinant DNA into Recipient Cell
- 5. Recombinant Screening
- 6. Cloned Genes Expression
- Final examination
Taught by
Su Wenhui, Liu Baoqin, and Hu Bo
