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XuetangX

Molecular Medicine Techniques

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Overview

Molecular Medicine is a subject that studies the nature of life at molecular level. Techniques in Molecular Medicine have been introduced to all fields of modern biomedical research and are now finding their way into diagnosis and even therapy. It also is a highly practical subject, and plays a very important role in training the students with overall quality and innovative ability.

The purpose of these serial videos is to introduce the techniques used in medicine laboratories for undergraduate students and ensure that they can master the fundamental laboratory skills necessary for their future researches.

A wide range of topics, including protein expression and purification, enzyme kinetics, purification and isolation of DNA, and polymerase chain reaction, are described for the immediate application in laboratory. With these videos, students can get as close as possible to do an experiment in the laboratory. As a result, students are expected to be familiar with the general principles behind each experiment from the lecture.


Syllabus

  • Chapter 1 The Extraction of Genomic DNA
    • Chapter 2 Isolation of Plasmid DNA
      • Chapter 3 Polymerase Chain Reaction (PCR)
        • Chapter 4 Lowry method for protein determination
          • Chapter 5 Protein Relative Molecular Weight Determination by Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis(SDS-PAGE)
            • Chapter 6 Glutathione-S-transferase fusion protein purification with Affinity chromatography
              • Chapter 7 Determination of Km value of alkaline phosphatase
                • 测试

                  Taught by

                  Jinzhou Medical University

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