Explore a comprehensive 12-hour tutorial on biological techniques covering a wide range of chromatography methods, microscopy techniques, and advanced analytical procedures. Delve into various chromatography types, including gas chromatography, HPLC, affinity chromatography, and gel filtration. Learn about fluorescence techniques such as FLIP and FRAP, and understand centrifugation methods like isopycnic and zonal ultracentrifugation. Discover microscopy principles, including numerical aperture, resolution, and various types like polarizing, phase contrast, and dark field microscopy. Examine molecular biology techniques such as the yeast two-hybrid system, protein tagging, and nucleic acid hybridization. Study advanced analytical methods like X-ray crystallography, mass spectrometry, and proteomics. Gain insights into DNA analysis techniques, including restriction fragment length polymorphism, Sanger sequencing, and comparative genomic hybridization. Master gel electrophoresis techniques and explore cutting-edge topics in metabolomics and flow cytometry.
Overview
Syllabus
CHROMATOGRAPHY PART 1.
CHROMATOGRAPHY PART 2.
Chromatogram.
Gas chromatography.
HPLC chromatography principle and instrumentation.
Affinity chromatography.
Protein purification | protein dialysis.
Fluorescence loss in photobleaching or FLIP.
Fluorescence recovery after photobleaching | FRAP.
Gel filtration chromatography.
Hydropathy plot.
Ion exchange chromatography.
Isopycnic centrifugation.
Mobility of phospholipid.
Zonal ultracentrifugation.
Numerical aperture of a microscope.
Resolution of a microscope.
Polarizing microscopy.
Phase contrast microscopy.
Yeast two hybrid system.
Aberration of microscopic lenses | chromatic aberration, spherical aberration and coma effect.
X ray crystallography basics explained.
Protein tagging.
Isoelectric focusing in 2d gel electrophoresis.
Hybridoma.
Dark field microscopy.
DIC microscopy.
FRET - fluorescence resonance energy transfer (fret).
Nucleic acid hybridization | DNA hybridization.
Serological tests.
Nucleotide probe hybridization.
Nucleotide probe hybridization.
Restriction endonuclease.
Restriction endonuclease.
Restriction fragment length polymorphism.
Restriction fragment length polymorphism.
Sanger sequencing method of DNA | chain termination method.
Centrifugation.
Mass spectrometry part 5: Graph analysis.
AFLP.
RAPD.
Spectral karyotyping.
Comparative genome mapping.
Comparative genomic hybridization.
Microdisection of chromosome.
Metabolomics.
Proteomics.
Trypsin digestion.
Mass spectrometry part 9 time of flight analyzer.
Mass spectrometry part 8 quadruple analyzer.
Mass spectroscopy part 7 MALDI and ESI.
Mass spectroscopy part 6 Ionization in Mass spec.
Metastable ion.
Silver staining.
Mclafferty rearrangement problems 2.
Mclafferty rearrangement problems 1.
Mclafferty rearrangement.
2 D gel electrophoresis.
Flow Cytometry.
Taught by
Shomu"s Biology
