Overview

Genetic engineering is one of the significant courses in biology related disciplines (bioengineering, biotechnology, bioscience, etc.) and a key subject of life science. It involves both theoretical and experimental knowledge of biochemistry, microbiology, molecular biology and some other disciplines. And the course mainly aims to educate the students to understand the significance of genetic engineering, the basic theory, technology and application of genetic engineering operation, and meanwhile to lay a theoretical foundation for future genetic engineering research.

Genetic engineering is an emerging subject of biotechnological science, which was born in 1970s on the basis of the comprehensive development of molecular biology and molecular genetics. The establishment and development of it depend directly on the development and progress of gene and molecular biology, which marks a close and inseparable internal relationship between them. Through the teaching of genetic engineering, we aim to achieve the following objectives: To help students understand the basic concepts and principles of genetic engineering; To enable students to master the main technical principles and applications of genetic operations; To enable students to master the research content and operation process of genetic engineering; To help students master the basic experimental techniques of genetic engineering.

The course mainly includes the basic principles and methods of genetic engineering; The obtaining of target genes; Tool enzymes and vectors of gene cloning; Identification and expression of recombiner; Gene sequencing technology; Application of genomics and genetic engineering.

Syllabus

Chapter 1 Introduction and Basic Knowledge

1.1 Introduction and Basic Concepts - What Makes the Blue Blooms of Roses - Let Us Start to Learn about the Genetic Engineering
1.2 History - How Has Genetic Engineering Come into Being
1.3 Basis of Molecular Biology

Chapter 2 Preparation of Target Genes

2.1 Nucleic Acid Extraction - How to Get DNA in an Organism - Nucleic Acid Extraction Tells You the Truth
2.2 Nucleic Acid Detection - How to Confirm Whether DNA Has Been Obtained
2.3 PCR Principle - A Good Way to Increase DNA
2.4 PCR Influencing Factors - Increase DNA by Experiments
2.5 Reverse Transcription PCR and the Construction of cDNA Library
2.6 The Genome-Wide Amplification and Genome Research
2.7 Tool Enzyme - Scissors and Glue in Genetic Engineering - Tool Enzyme

Chapter 3 Plasmid

3.1 Blue-White Screening - Fun Blue-White Screening - Strain Screening Method Based on Colors
3.2 Plasmid Vector - Who Helps the Scientists Complete the Genetic Modification
3.3 Viral Vector - Introduce more DNA into Plasmid Vectors to Prepare for Later Sequencing

Chapter 4 Introducing Target Gene into Recipient Cells

4.1 Recipient Cells - Selfless Cells in Organisms
4.2 Transformation - The Way to Introduce Selected Genes into Cells
4.3 Screening and Identification of Recombiner - Screening and Identification of Specially Processed Substances
4.4 DNA Sequencing Technology

Chapter 5 Expression of Exogenous Gene

5.1 Prokaryotic Expression System
5.2 Eukaryotic Expression System

Chapter 6 Application of Genetic Engineering

6.1 Application of Genetic Engineering-Industrial
6.2 Application of Genetic Engineering-Agriculture
6.3 Application of Genetic Engineering-Medicine
6.4 Gene Editing - A Technology that Can Treat Diseases

Chapter 7 Debating on Genetic Engineering and Biosafety

7.1 Discussions on Genetic Engineering and Biosecurity - Genetic Engineering Is a Double-Edged Sword

Chapter 8 Experiments

Experiment 1 Extraction and Detection of Genomic DNA of Escherichia Coli
Experiment 2 PCR Amplification, Capillary Electrophoresis and Gel Extraction of Chorismate Lyase Gene
Experiment 3 Cloning, Ligation and Transformation of Genes
Experiment 4 Plasmid Extraction and Positive Clone Detection