In today’s world, genetic engineering plays an important role in drug discovery and pharmaceutical industries. Various hormones, proteins, interferons, antibodies and other therapeutics can be produced by introducing rDNA/foreign gene to the host organism. Genetic engineering can also be used for controlling various environmental factors like air, water, and soil pollution. It also plays a major role in waste management and bio remediation. Using this technique, the resistant varieties of crops can be developed with yield enhancement. After successful completion of the course, students will be able to learn the basic concepts of genetic engineering, its tools and techniques, cloning, applications of cloning, recent advancements and about transgenic plants and animals. The students will be able to think critically and apply their knowledge in conducting research projects.
Genetic Engineering and Its Recent Advancements
IGNOU and Chhattisgarh Swami Vivekanand Technical University via Swayam
Overview
Syllabus
Week
Module
Titles
Week 1
Module I: Fundamentals of Genetic Engineering
Introduction to Genetic Engineering
Tools of Genetic Engineering
Steps involved in Genetic Engineering
Week 2
Module II: Cloning, Types of Vectors and Genetic Libraries
Principles of cloning
Applications of cloning
Week 3
Different types of libraries
Library Screening
Week 4
Cloning and Expression vectors
DNA replication
Rolling circle model
Theta replication model
Bacterial Artificial Chromosome, Yeast Artificial Chromosome
Mammalian Artificial Chromosome
Week 5
Module III: Introduction of DNA into host
Genetic Manipulation of cells
Types of Transfection
Genetic manipulation in plants
Transgenic Plants
Transgenic Animals
Week 6
Module IV:
Gene silencing and regulation of Gene expression
Gene silencing
m RNA, si RNA, RNAi
Knock out technology
Week 7
Gene Expression
Regulation of Gene Expression in prokaryotes
Transcription and its regulation in prokaryotes
Translation and its regulation in prokaryotes
Week 8
Module V: Restriction modification system and Applications of Genetic Engineering
Restriction Endonucleases and its Types
Restriction modification system
Reporter genes
Microarrays
DNA foot printing
Week 9
Gene Therapy
Future trends in transgenic plants
Future trends in transgenic animals
Pharm animals
Week 10
Module VI: Genome Editing
Genome editing
CRISPR-Cas technology
Week 11
Mechanism of CRISPR-Cas
Case studies of CRISPR-Cas
Week 12
Basic instrumentations in Genetic Engineering
Sequence analysis
Taught by
Dr. Tanushree Chatterjee & Dr. Sharmistha Banerjee
