Molecular biology is a subject that studies phenomena and fundaments of life at molecular level. It elucidates the essence of life phenomenon and the knowledge of structure and function of biological macromolecules. The new theories and technologies have facilitated the development of molecular biology, and promoted the development of life science and medical science in various aspects. The contents of this course includes introduction, genes, genome and genomics, regulation of gene expression, molecular mechanism of DNA damage and repair, basic methods of gene structure and expression analysis, gene cloning and gene expression in vitro, proteomics research methods, The molecular basis of diseases production, the principle and application of gene diagnosis, and the principle and application of gene therapy. This course aims to help students to master the basic theories and skills of molecular biology in a more comprehensive manner, to enable them to understand and analyze the life phenomenon at molecular level, and to elucidates the pathogenesis of diseases at molecular basis, and to understand the applications of these knowledge in the frontier of life sciences and medical sciences. By studying our course, we anticipate the students not only to gain knowledge of molecular biology, but also learn to analyze and solve problems in a scientific manner.
Overview
Syllabus
- Week 1 （1.） Introduction
- 1.1 The research content of molecular biology
- 1.2 Important events in the development of molecular biology
- 1.3 Molecular Biology and Related Disciplines
- Week1 (2.) Genes and Genomes
- 2.1 The Concept and Structure of Genes
- 2.2 Prokaryotic genome
- 2.3 Eukaryotic genome
- 2.4 Viral genome
- Week2 Regulation of Gene Expression
- 3.1 The common characteristics of gene expression in prokaryotes and eukaryotes
- 3.2 Mechanism of gene expression regulation in prokaryotes
- 3.3 Characteristics and regulation of genomic DNA expression in eukaryotes
- 3.4 Regulation of eukaryotic gene expression at transcriptional and post-transcriptional level
- Week 3 Mechanisms of DNA Damage and Repair
- 4.1 Causes and Consequences of DNA Damage
- 4.2 Mismatch Repair and Direct Repair
- 4.3 Base Excision Repair and Nucleotide Excision Repair
- 4.4 Recombination Repair and SOS Repair System
- Week 4 ( 1.) Basic Methods of Gene Structure and Expression Analysis
- 5.1 DNA sequence analysis
- 5.2 Polymerase chain reaction
- 5.3 Real-time fluorescence quantitative PCR
- 5.4 Nucleic acid molecular hybridization techniques
- Week 4 ( 2.) Gene Cloning and Expression
- 6.1 Principal enzymes used in gene cloning
- 6.2 Vectors for Gene cloning
- 6.3 The principal process of gene cloning
- 6.4 The E.coli expression system
- Week 5 Proteomics research methods
- 7.1 The Concept and History of Proteomics
- 7.2 Research method of proteome expression pattern--Two-dimensional electrophoresis
- 7.3 Research method of proteome expression pattern-- Mass spectrometr
- 7.4 Research Methods of Proteome Functional pattern
- Week 6 (1. )Molecular basis of disease
- 8.1 Abormalities in genes and proteins are the molecular basis of diseases
- 8.2 Cell-related factors cause abnormal gene expression and cause disease
- Week 6 (2.) Gene diagnosis
- 9.1 Concept, Features and Strategies of Gene Diagnosis
- 9.2 Molecular Biology Techniques Commonly Used in Gene Diagnosis
- 9.3 Molecular Biology Techniques Commonly Used in Gene Diagnosis II
- 9.4 Gene Diagnosis of Sickle Cell Disease
- Week 7 Gene Therapy
- 10.1 Basic strategies for gene therapy
- 10.2 Gene transfer
- 10.3 Gene interference
- 10.4 Controlled expression of therapeutic genes
- 10.5 Application of gene therapy
- Final Exam
Taught by
Jing LIU, Zhaojun ZENG, Zhiyiong LUO, Shuming SUN, Haitao ZENG, Kunlu WU, Huiyong CHEN, Xiaojuan XIAO, Dehui XIONG, Min ZHU, and Jingping HU
