Selecting Between-sample ChIP-Seq Normalization Methods - Assumptions and Implications
Computational Genomics Summer Institute CGSI via YouTube
Overview
Syllabus
Intro
High throughput sequencing
What is ChIP sequencing
Differential binding
RNAseq
How much does normalization matter
Library size
Background bins
Road map
Capital T truth
Factoring
spiking controls
symmetry
Asymmetry
Size Factors
Relative Log Expression
Technical Conditions
Simulations
False Discovery Rate
Symmetrical DNA Binding
Background Bin
Entire Genome
Four Images
Technical Condition
Trend
Data
Principal Components
Summary
Controls
Biological Experiment
Conclusion
Taught by
Computational Genomics Summer Institute CGSI