Overview
Learn essential medical laboratory techniques in this comprehensive course covering four key modules: cell culture, gene detection, protein analysis, and advanced instrumentation. Master cell culture techniques including growth characteristics, preparation procedures, contamination prevention, and transfection methods. Explore gene detection methodologies from nucleic acid extraction to PCR techniques, gel electrophoresis, DNA sequencing, and transcriptome analysis. Develop expertise in protein analysis through radioimmunoassay, ELISA, immunohistochemistry, and protein interaction studies. Gain hands-on experience with sophisticated laboratory equipment including flow cytometry, live cell imaging stations, laser microdissection systems, capillary electrophoresis, and animal behavior analysis platforms. Perfect practical skills essential for medical research and clinical laboratory work through detailed theoretical instruction and laboratory applications.
Syllabus
- 第一模块 细胞培养
- 1.1 细胞培养技术
- 1.2 体外培养细胞的生长和增殖特点
- 1.3 细胞培养的准备工作
- 1.4 细胞的处理
- 1.5 细胞的污染
- 1.6 细胞转染技术
- 1.7 细胞活力相关测定
- 第二模块 基因检测技术
- 2.1 基因检测相关技术和原理
- 2.2 核酸的提取与分离
- 2.3 聚合酶链式反应
- 2.4 琼脂糖凝胶电泳
- 2.5 荧光定量PCR
- 2.6 DNA测序
- 2.7 转录组研究技术
- 2.8 转录组测序技术
- 第三模块 蛋白质相关测定
- 3.1 蛋白质测定常用的实验方法
- 3.2 放射性免疫分析
- 3.3 酶联免疫吸附
- 3.4 免疫组化
- 3.5 蛋白质相互作用的研究方法
- 3.6 核酸和蛋白质相互作用的研究方法
- 第四模块 大型仪器平台的应用
- 4.1 流式细胞术
- 4.2 活细胞工作站
- 4.3 激光显微切割系统
- 4.4 毛细管电泳
- 4.5 小动物高通量行为分析系统
- 4.6 小动物活体成像系统
- 4.7 多功能实验动物监测系统
- 期末考试
Taught by
zhang huaping, Pengfei Li, Zhenxiang Zhao, Liu Yiqian, Zhou Hui, Haixia Yu, Yang Jian , Yin Yiru, and Fang Wang