高通量DNA测序技术

Learn about high-throughput DNA sequencing technology through this comprehensive course covering fundamental principles to advanced applications. Begin with an introduction to the significance of high-throughput DNA sequencing before diving into sequencing chemistry, including synthesis methods and nucleotide marking techniques. Master genome sample processing and amplification technologies, exploring nucleic acid isolation, library preparation, emulsion PCR, and bridge PCR. Study sequencing library preparation techniques including coded libraries, single-cell sequencing, and spatial transcriptomics. Examine the structure and operating principles of second-generation sequencing instruments, focusing on fluorescence excitation, CCD imaging devices, microfluidic chips, and integrated sequencing chips. Explore third-generation methods including single-molecule imaging and nanopore array sequencing. Discover practical applications in scientific research, DNA storage technology, and personalized medicine, including uses in pathogenic microorganism research, tumor gene detection, and disease susceptibility gene testing.