Learn the safety protocols, operating principles, and practical applications of major biomedical instruments through this comprehensive course. Master laboratory safety regulations before diving into detailed explorations of transmission electron microscopy (TEM), including FEI Talos F200C operations and sample preparation techniques. Explore laser confocal microscopy with hands-on training on LSM780 and LSM880 two-photon systems, along with high-content imaging analysis. Study flow cytometry through both analytical and cell sorting applications. Gain practical experience with animal imaging and behavioral analysis, including small animal optical in-vivo imaging, X-ray irradiation, and behavioral analysis systems. Conclude with advanced multi-factor detection methods, covering multimarker microplate detection and cellular energy metabolism analysis systems.
Overview
Syllabus
- 前言 大型实验仪器器材概述
- 第一章 实验室安全规范
- 1.1 实验室安全（一）
- 1.2 实验室安全（二）
- 1.3 实验室安全（三）
- 第二章 透射电镜
- 2.1 透射电镜的组成
- 2.2 透射电镜样品观察-FEI Talos F200C基本操作及应用
- 2.3 透射电镜样品制备-常规超薄切片技术
- 2.4 透射电镜在生物医学上的应用
- 2.5 透射电镜常规样品制备
- 第三章 激光共聚焦显微镜
- 3.1 激光共聚焦显微镜原理及LSM780的应用
- 3.3 双光子共聚焦显微镜LSM880应用
- 3.4 激光共聚焦高内涵成像分析系统的应用
- 第四章 流式细胞仪
- 4.1 流式细胞分析仪的应用
- 4.2 流式细胞分选仪的应用
- 第五章 动物影像及行为学
- 5.1 小动物光学活体成像技术原理及应用
- 5.2 X线辐照仪的应用
- 5.3 动物行为学分析系统的应用
- 第六章 多因子检测
- 6.1 多标记微孔板检测系统的应用
- 6.2 细胞能量代谢分析系统的应用
- 期末考试
Taught by
Shangming Liu, , , , , , , Wanghao, and
