Learn practical techniques for using isotope tracers in biomedical research through this comprehensive course. Master fundamental principles of isotope tracing technology, including its significant contributions to biomedical development and various applications. Gain hands-on experience with radioactive biomedical experimental operations, covering design principles, radiation protection, and waste management protocols. Explore the preparation and quality control of common radioactive probes, including molecular probe synthesis, purification, and identification methods. Practice in vitro and in vivo tracer experiments, understanding receptor radioanalysis techniques and lymphocyte proliferation measurements. Discover small animal positron emission imaging experimental procedures, including PET principles, applications in biomedical research, and equipment operation protocols. Develop essential skills through practical laboratory sessions and end-of-course assessments to effectively apply isotope tracing techniques in biomedical research settings.
Overview
Syllabus
- 第一讲 同位素示踪技术在生物医学研究中的应用
- 同位素示踪技术概论
- 1-1 同位素示踪技术对生物医学发展的贡献
- 1-2 同位素示踪技术基本原理
- 1-3 同位素示踪技术应用概述
- 第二讲 放射性生物医学实验操作要领
- 2-1 放射性生物医学实验设计原则
- 2-2 放射性操作防护原则
- 2-3 放射性生物医学实验操作注意事项
- 2-4 放射性实验废物处理
- 第三讲 常见放射性标记探针制备及质量控制
- 3-1 放射性标记分子探针制备原则及分类
- 3-2 放射性碘标记分子探针制备
- 3-3 放射性锝标记分子探针及正电子核素分子探针的制备
- 3-4 放射性放射性标记化合物的纯化和鉴定
- 第四讲 放射性标记探针体外示踪实验操作
- 4-1 放射性体外示踪实验基本原理
- 4-2 受体放射性分析技术
- 4-3 3-H-TdR掺入测定淋巴细胞增殖实验
- 第五讲 放射性标记探针体内示踪实验操作
- 5-1 放射性体内示踪实验基本原
- 5-2 物质吸收的示踪实验
- 5-3 物质分布与转运的示踪实验
- 5-4 生物活体放射性自显影显像
- 第六讲 小动物正电子核素显像实验操作
- 6-1 正电子发射计算机断层显像原理
- 6-2 小动物PET在生物医学研究应用概述
- 6-3 小动物PET仪器结构及操作流程
- 期末测试
Taught by
Ting Liang, Guihua Hou, and Gao Feng
