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Peking University

细胞培养技术

Peking University via XuetangX

Overview

Learn essential cell culture techniques through this comprehensive Chinese language course, covering fundamental principles and practical laboratory methods. Master key procedures including cell adhesion culture, material preparation, cell recovery, media changes, cell passage, cryopreservation, and cell counting. Explore primary cell culture techniques with detailed instruction on experimental animal preparation, tissue collection, tissue inoculation, and result observation. Progress through structured units with self-assessment opportunities and gain hands-on experience in both basic and advanced cell culture methodologies used in biological research and biotechnology applications.

Syllabus

  • 第一章 细胞培养总论
    • 细胞培养总论
    • 单元测验
  • 第二章 贴壁细胞培养
    • 第一节 实验材料准备
    • 第二节 细胞复苏
    • 第三节 细胞换液
    • 第四节 细胞传代
    • 第五节 细胞冻存
    • 第六节 细胞计数
    • 单元测验
  • 第三章 原代细胞培养
    • 第一节 实验动物准备
    • 第二节 组织取材
    • 第三节 组织接种
    • 第四节 实验结果观察
    • 单元测验
  • 期末考试

    Taught by

    Bai Yun, Yang Hua, CAO Jixiang, and Song Qing

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