Dive into the fundamental concepts of molecular biology with this comprehensive video series. Explore the central dogma of molecular biology, including DNA replication, transcription, and translation. Learn about DNA polymerase proofreading, nucleotide addition during DNA synthesis, and the roles of clamp loaders and sliding clamps. Discover the intricacies of DNA methylation, replication initiation, lagging strand synthesis, and termination in E. coli. Understand DNA footprinting experiments and DNase footprinting techniques. Delve into transcription processes, RNA polymerase details, and RNA processing. Examine protein synthesis in prokaryotes and the lac operon concept and regulation. Investigate various DNA replication mechanisms, including rolling circle replication, D-loop replication, and the pan handle model. Study plasmid replication, copy number control, and regulation by cop genes and iterons. Gain insights into multiple copy number plasmids, low copy number plasmids, and copy number control of F plasmids.
Overview
Syllabus
Central dogma (replication, transcription and translation).
DNA replication 1 | process of DNA duplication.
DNA polymerase proofreading.
DNA polymerization | nucleotide addition during DNA synthesis.
Clamp loader and sliding clamp.
DNA clamp loader and primase.
DNA polymerization by DNA polymerase 1.
DNA methylation in DNA replication regulation.
DNA Replication initiation.
Lagging strand synthesis.
Termination of DNA replication in E.coli.
DNA footprinting experiment.
DNase footprinting.
TRANSCRIPTION 01.
RNA polymerase detail.
RNA processing.
Protein synthesis in prokaryotes.
Protein synthesis process.
Lac operon concept class 12.
Lac operon repression - lac repressor.
Lac operon regulation.
DNA replication initiation in E coli.
DNA rolling circle replication.
Rolling circle replication advanced.
D loop replication.
Pan handle model of DNA replication.
Replication of single stranded plasmids.
Copy number control in plasmids.
Copy number regulation by cop genes.
Multiple copy number plasmids.
Low copy number plasmids.
Iteron mediated copy number control.
Copy number control of f plasmids.
Taught by
Shomu"s Biology
