Explore cutting-edge genomics technologies and their applications in elucidating principles of gene regulation in mammalian cells through this 54-minute seminar by Bas van Steensel from the Netherlands Cancer Institute. Delve into an innovative approach for efficiently altering the linear structure of large genomic loci in a highly multiplexed manner, and examine its impact on gene regulation and spatial chromatin organization. Learn about enhancer-promoter interactions, the effects of gene and regulatory element locations on function, and scalable DNA element manipulation techniques. Investigate the formation of strong barriers for activation by specific control regions and the role of loop extrusion in gene regulation. Gain insights into lamina-associated domains (LADs) and explore more scalable strategies for understanding genome organization and function.
Probing Principles of Genome Organization and Function
Center for Physical Genomics and Engineering via YouTube
Overview
Syllabus
Monthly Seminar on Physical Genomics
Probing principles of genome organization and function
How do Enhancers select their target Promoter
Combinatorial EP reporter assay
Testing for selectivity
Does compatibility make enhancers and promoter loop more frequently?
How does location of genes and regulatory elements affect function?
Scalable "hopping" of DNA elements
Sox2 gene forms a strong barrier for activation by SCP
Does loop extrusion start from the SCR, proceed to the left catch the first gene it encounters?
Lamina associated domains (LADs)
A more scalable strategy?
Taught by
Center for Physical Genomics and Engineering
