Building Amplification-Free CRISPR/Cas Biosensors Through Autocatalysis Reaction Induced by Circular DNA Nanostructure

Watch a 31-minute webinar presentation by Dr. Yi Li, a Research Associate at the University of New South Wales, exploring innovative developments in CRISPR/Cas biosensor technology. Learn about a groundbreaking Autocatalytic CRISPR/Cas12a Amplification Reaction (AutoCAR) system that manipulates Cas12a activity using circular DNA nanostructure. Discover how this novel approach achieves 1 attomole sensitivity in nucleic acid detection at room temperature without requiring additional amplifications. Understand the system's potential for detecting DNA/RNA targets from H. pylori and SARS-CoV-2 genomes at approximately 1 copy/μL. Gain insights into how this development expands the possibilities of CRISPR/Cas biotechnology for applications in biochemistry, bioanalysis, and diagnostics, demonstrating significant improvements over traditional Cas12a ribonucleoprotein (RNP) methods.