Molecular Diagnosis Enabled by Dynamic DNA Assembly and CRISPR Cas Toolbox

Explore groundbreaking developments in molecular diagnostics through this 33-minute webinar presented by Professor Feng Li from Sichuan University's College of Chemistry. Discover how CRISPR-based diagnostics (CRISPR-Dx) combined with dynamic DNA assembly are revolutionizing disease detection with unprecedented sensitivity, specificity, and simplicity. Learn about innovative approaches including a plasmonic CRISPR Cas12a assay for detecting grapevine viral infections, a molecular translator method for protein biomarker detection, and the development of ultrasensitive protein assays capable of identifying attomolar concentrations of SARS-CoV-2 antibodies in challenging clinical samples. Gain insights into a novel CRISPR-like assay utilizing RNA-cleaving DNAzyme coupled with isothermal sequence and signal amplification, offering enhanced accessibility and multiplexing capabilities beyond traditional CRISPR-DX methods.