ABOUT THE COURSE:This course will start with Basic principles of NMR, Discuss practical aspects of NMR spectroscopy, peptide NMR, Protein NMR, Nucleic acids NMR. We will teach with examples how to solve structure and measure dynamics using NMR.PRE-REQUISITES: Under graduate level understanding of Physics and Mathematics, Basic course on NMR spectroscopy.INTENDED AUDIENCE: M.Sc./ PhD students & Scientist, and Scientists working in Biopharma Industries.INDUSTRY SUPPORT: Biocon, Sunpharma, Alembic, Bharat Biotech, Aurobindo Biopharma etc.
Overview
Syllabus
Week 1:Basics Principles of NMR; Nuclear Spin in static magnetic field
Week 2:Basics Principles continued; Chemical Shift; Factors Influencing Isotropic Chemical shifts
Week 3:NMR instrumentation and Peptide sample preparation; Overview of NMR instrument Magnet, transmitters etc
Week 4:Two-dimensional NMR And Practical Aspects for experiment; Two- dimensional correlation experiments COSY
Week 5:Peptide and Protein structure Structural features and parameters; Structural features and parameters
Week 6:Sample preparation for Protein NMR; Expression systems, Optimization of protein expression
Week 7:Multi-dimensional Heteronuclear NMR experiments; 2D Heteronuclea r NMR experiments
Week 8:Multi-dimensional Heteronuclear NMR experiments; Experiments for Backbone assignment
Week 9:Protein Structure determination from NMR data; Structure calculation methods and NMR parameters
Week 10:Protein dynamics from NMR data; Theory of spin relaxation in proteins Week 11:Nucleic acids NMR experiments; Structural features and parameters for Nuclei acids Week 12:DNA and structure determination from NMR data
Week 9:Protein Structure determination from NMR data; Structure calculation methods and NMR parameters
Week 10:Protein dynamics from NMR data; Theory of spin relaxation in proteins Week 11:Nucleic acids NMR experiments; Structural features and parameters for Nuclei acids Week 12:DNA and structure determination from NMR data
Taught by
Prof. Ashutosh Kumar, Prof. R. V Hosur
