About the Course:In this course, after introducing NMR and the internal interaction parameters, I will discuss some advanced concepts and techniques, such as, relaxation phenomenon and their measurements, NOE and ROE, spin system nomenclature, quantum mechanical analysis of spectra, the polarization transfer mechanism, chemical exchange, pure shift NMR, diffusion ordered spectroscopy, spectra of many heteronuclei, two dimensional NMR and routine 2D experiments, product operator formalism, analysis of 2D spectra. The multiple quantum techniques, the solid state NMR, magic angle spinning and cross polarization, CPMAS techniques and solid state NMR experiments will be discussed in detail.INTENDED AUDIENCE: Postgraduates and Research StudentsPREREQUISITES: Basic knowledge of NMR Spectroscopy and Analysis of SpectraINDUSTRY SUPPORT: All pharma industries which have NMR facilities
Advanced NMR Techniques in Solution and Solid-State
Indian Institute of Science Bangalore and NPTEL via Swayam
Overview
Syllabus
Week 1:NMR Introduction, NMR Concepts, Internal Interaction, Chemical Shifts, Internal Interaction, J Couplings, Fourier Transforms and 1D spectraWeek 2:Pulse phase and Receive phase, Evolution of chemical shifts, Evolution of Scalar Couplings, Concept of Relaxation, Relaxation MechanismsWeek 3:T1 and T2 measurements, Nuclear Overhauser Effect, NOE and ROE, Coupled Spin Systems Nomenclature, Weakly and strongly coupled two spin systems, energy levels and transitionsWeek 4:Weakly and strongly coupled two spin systems, energy levels and transitions , Three coupled spin systems, energy levels and transition frequencies, Spin Systems with more than three coupled spins, Quantum Mechanical Analysis of Two Weakly Coupled Spectra, Quantum Mechanical Analysis of AB SpectraWeek 5:Pure Shift NMR, 2D Pure Shift Experiments, Chemical Exchange, Chemical Exchange, Spin EchoesWeek 6:Polarization Transfer Techniques, Polarization Transfer Techniques, 13C NMR , 13C NMR, Spectra of HeteronucleiWeek 7:Spectra of Heteronuclei, Spectra of Heteronuclei, Phase cycling, Pulse Field Gradients, Diffusion Ordered Spectroscopy Week 8:Diffusion Ordered Spectroscopy, Shaped Pulses spin lock, coherence order, Shaped Pulses spin lock, coherence order, 2D NMR Concepts COSY, TOCSY pulse SequencesWeek 9:"Modified COSY sequences HSQC, NOESY Pulse Sequences Homo and heteronuclear Resolved spectra Analysis of 2D COSY and HSQC spectra 2D Experimental aspects"Week 10:Product Operators: Evolution of coherences, Product Operators: Evolution of coherences, Product Operators: Evolution of coherences, Concept of Multiple Quantum NMR, Multiple Quantum Spectra of scalar and dipolar coupled spinsWeek 11:Difference between Solid state and solution state NMR , Anisotropic NMR parameters: CSA and dipolar couplings, Magic Angle Spinning, Magic Angle Spinning, Cross Polarization Week 12:CPMAS, High Speed CP, Suppression of Spinning side bands, Selected Experiments in Solid state NMR, Selected Experiments in Solid state NMR
Taught by
Prof. N. Suryaprakash
