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How to choose the optimal fluorophore and filter?
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Tips and Tricks for Successful Multiplexing IHC
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- 1 Intro
- 2 Multiplexed immunohistochemistry (mIHC)
- 3 Optimization of sample preparation
- 4 Blocking autofluorescence
- 5 Tips for blocking background
- 6 How to plan a successful multiplexed IHC experiment
- 7 How to choose an appropriate primary antibody?
- 8 The Thermo Fisher Scientific antibody search tool
- 9 Primary antibody protocol optimization
- 10 How do you choose an appropriate secondary antibody?
- 11 Secondary antibody protocol optimization
- 12 Types of labeling strategies
- 13 How to decide appropriate labeling strategy?
- 14 Direct conjugates of primary antibody
- 15 Standard secondary antibody labeling
- 16 Avidin-biotin amplification
- 17 Enzyme amplification
- 18 Points to remember for labeling strategy
- 19 Multiplex fluorescence: The main colors and beyond...
- 20 How to choose the optimal fluorophore and filter?
- 21 Spectral overlap and multiplexing
- 22 Fluorophore photobleaching and photostability
- 23 Controls and multiplexing
- 24 Some important rules to remember for multiplexed IHC
