Tips and Tricks for Successful Multiplexing IHC

Tips and Tricks for Successful Multiplexing IHC

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Intro

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1 of 24

Intro

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Tips and Tricks for Successful Multiplexing IHC

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  1. 1 Intro
  2. 2 Multiplexed immunohistochemistry (mIHC)
  3. 3 Optimization of sample preparation
  4. 4 Blocking autofluorescence
  5. 5 Tips for blocking background
  6. 6 How to plan a successful multiplexed IHC experiment
  7. 7 How to choose an appropriate primary antibody?
  8. 8 The Thermo Fisher Scientific antibody search tool
  9. 9 Primary antibody protocol optimization
  10. 10 How do you choose an appropriate secondary antibody?
  11. 11 Secondary antibody protocol optimization
  12. 12 Types of labeling strategies
  13. 13 How to decide appropriate labeling strategy?
  14. 14 Direct conjugates of primary antibody
  15. 15 Standard secondary antibody labeling
  16. 16 Avidin-biotin amplification
  17. 17 Enzyme amplification
  18. 18 Points to remember for labeling strategy
  19. 19 Multiplex fluorescence: The main colors and beyond...
  20. 20 How to choose the optimal fluorophore and filter?
  21. 21 Spectral overlap and multiplexing
  22. 22 Fluorophore photobleaching and photostability
  23. 23 Controls and multiplexing
  24. 24 Some important rules to remember for multiplexed IHC

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