Completed
The Thermo Fisher Scientific antibody search tool
Class Central Classrooms beta
YouTube videos curated by Class Central.
Classroom Contents
Tips and Tricks for Successful Multiplexing IHC
Automatically move to the next video in the Classroom when playback concludes
- 1 Intro
- 2 Multiplexed immunohistochemistry (mIHC)
- 3 Optimization of sample preparation
- 4 Blocking autofluorescence
- 5 Tips for blocking background
- 6 How to plan a successful multiplexed IHC experiment
- 7 How to choose an appropriate primary antibody?
- 8 The Thermo Fisher Scientific antibody search tool
- 9 Primary antibody protocol optimization
- 10 How do you choose an appropriate secondary antibody?
- 11 Secondary antibody protocol optimization
- 12 Types of labeling strategies
- 13 How to decide appropriate labeling strategy?
- 14 Direct conjugates of primary antibody
- 15 Standard secondary antibody labeling
- 16 Avidin-biotin amplification
- 17 Enzyme amplification
- 18 Points to remember for labeling strategy
- 19 Multiplex fluorescence: The main colors and beyond...
- 20 How to choose the optimal fluorophore and filter?
- 21 Spectral overlap and multiplexing
- 22 Fluorophore photobleaching and photostability
- 23 Controls and multiplexing
- 24 Some important rules to remember for multiplexed IHC
