Chromatin and Nucleosome Density: How DNA Damage Response Alters Chromatin Dynamics

Explore a comprehensive seminar on chromatin dynamics and DNA damage response presented by Susan Gasser. Delve into the intricate world of eukaryotic nucleosomes and their crucial role in maintaining genome integrity and chromatin organization. Discover recent findings from the Gasser group revealing how DNA breaks lead to histone eviction and a decrease in total nuclear histone levels. Examine the mechanisms behind histone degradation, including the involvement of the INO80 chromatin remodeler complex and the proteasome. Learn about the consequences of histone degradation, such as chromatin decompaction and increased fiber flexibility. Analyze quantitative mass spectrometry data showing significant changes in the chromatin-associated proteome during DNA damage response, including depletion of RNA polymerases and nucleosome remodelers. Investigate the increased association of ubiquitin ligases with chromatin and the role of INO80 in this process. Understand the physiological importance of histone degradation in DNA repair mechanics, particularly in homology search during homologous recombination. Gain insights into cutting-edge research in physical genomics and its implications for understanding DNA damage response mechanisms.