Explore a comprehensive 13-hour lecture series on molecular and cellular biology techniques. Dive into immunological methods, cytogenetic procedures, molecular markers, DNA sequencing technologies, and agricultural biotechnology. Learn about plasmid transfer, transgenic plant production, micro-RNA technology, and vaccination processes. Discover various molecular and cellular techniques essential for understanding protein and gene functions in biological sciences. Cover topics such as genetic markers, ELISA assays, immunofluorescence, PCR types, DNA repair mechanisms, next-generation sequencing, miRNA biogenesis, gene therapy approaches, vaccine development, and plant biotechnology. Gain insights into cutting-edge techniques like CRISPR, transgenic animal production, and knockout mice creation.
Molecular Techniques Lecture - Molecular Biology Techniques and Cell Biology Techniques
Overview
Syllabus
Genetic Markers | RAPD, RFLP, AFLP.
Sandwich ELISA assay.
ELISA test.
Antigens and antibodies.
Radial immunodiffusion.
Immunofluorescence.
Complement fixation test.
RFLP.
RAPD.
AFLP.
Quorum sensing in bacteria 3.
Quorum sensing in bacteria 2.
Quorum sensing in bacteria 1.
PCR types.
PCR (polymerase chain reaction) in detail.
Real time PCR.
Nucleotide excision repair.
Mismatch repair.
DNA repair mechanism - Base excision repair.
Next generation sequencing.
Maxam gilbert DNA sequencing method.
Illumina sequencing | DNA sequencing by synthesis.
DNA sequencing methods.
Exome sequencing.
miRNA biogenesis | microRNA mechanism.
shRNA mechanism | short hairpin RNA mediated knockdown and gene silencing.
RNAi mechanism | RNA interference pathway using siRNA and shRNA.
siRNA mechanism | short interfering RNA mediated gene silencing.
HIV life cycle.
In vivo gene therapy.
Gene therapy basics.
Ex vivo gene therapy.
Gene therapy using retrovirus vector.
Gene therapy using adeno virus.
Gene therapy using adeno associated virus.
HIV vaccine.
Cancer immunotherapy.
Vaccines part 7 - viral vaccine.
Vaccines part 6 - DNA vaccine.
Vaccines part 5 - conjugated vaccine.
Vaccines part 4 - killed vaccine.
Vaccines part 3 - live attenuated vaccines.
Vaccines part 2 - properties of vaccine.
Vaccines part 1 - what is vaccination.
Plant biotechnology 6.
Plant biotechnology 5.
Plant biotechnology 4.
Plant biotechnology 3.
Plant biotechnology 2.
Plant biotechnology 1.
Transgenic animals.
Knockout mice.
Transgenic mice.
Taught by
Shomu"s Biology
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Reviews
4.0 rating, based on 1 Class Central review
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I felt like some of the material was a little difficult to understand. The downside of this class was not being able to sit in a classroom environment and speak with an instructor to ask questions and receive feedback. Although I had the option of email, I would need an answer at that time and did not have the option of waiting (since I was only able to use weekends to log on and complete classwork).
The part of the class I really liked was having the flexibility of logging on at my convenience to do homework. I am generally a night owl and being able to log on at 11 or 12 at night was great for me!! I also like the course notes given by the instructor. They were easy to follow and very informative.
