Discover essential tips and techniques for accurate and reproducible pipetting in this 47-minute webinar presented by Kate Morgan, an experienced professional in liquid handling. Learn about different pipetting techniques and their impact on results, how to choose the right pipettes, and adjust your approach for various liquids and conditions. Explore the importance of regular maintenance and calibration to ensure reliability and compliance with ISO8655 standards. Gain insights into forward pipetting, reverse pipetting, multi-dispensing, and sequential dispensing modes, as well as strategies for handling viscous, foaming, and volatile liquids. Understand common sources of pipetting errors and how to mitigate them, including pre-wetting, uneven piston movement, and temperature effects. Perfect for daily pipette users, this webinar offers valuable knowledge to improve your laboratory skills and enhance the quality of your analytical experiments.
Get Pipetting Right - Tips and Tricks for Accurate and Reproducible Results
Overview
Syllabus
Intro
Which one is accurate?
Summary-Accuracy and Precision
Manufacturer's Specifications, Tacta Mechanical Pipette
Leaky Piston and Cylinder System
Pipette and Tip Compatibility-Performance
The Importance of Correct Pipetting Techniques
Pipetting Modes - Forward Pipetting
Pipetting Modes - Reverse Pipetting
Sources of Pipetting Error according to ISO 8655-2
Pre-wetting - Upto 2% Error
Uneven Piston Movement - Up to 0.5%
Pipetting Modes - Multi-dispensing and Sequential Dispensing
Pipetting Modes Dilution
The Best Techniques for Viscous Liquids
The Best Techniques for Foaming Liquids
Liquid temperature
Volatile Liquids
Mechanical pipettes require experience and training
Taught by
Labroots
