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Classroom Contents
Get Pipetting Right - Tips and Tricks for Accurate and Reproducible Results
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- 1 Intro
- 2 Which one is accurate?
- 3 Summary-Accuracy and Precision
- 4 Manufacturer's Specifications, Tacta Mechanical Pipette
- 5 Leaky Piston and Cylinder System
- 6 Pipette and Tip Compatibility-Performance
- 7 The Importance of Correct Pipetting Techniques
- 8 Pipetting Modes - Forward Pipetting
- 9 Pipetting Modes - Reverse Pipetting
- 10 Sources of Pipetting Error according to ISO 8655-2
- 11 Pre-wetting - Upto 2% Error
- 12 Uneven Piston Movement - Up to 0.5%
- 13 Pipetting Modes - Multi-dispensing and Sequential Dispensing
- 14 Pipetting Modes Dilution
- 15 The Best Techniques for Viscous Liquids
- 16 The Best Techniques for Foaming Liquids
- 17 Liquid temperature
- 18 Volatile Liquids
- 19 Mechanical pipettes require experience and training