Explore the principles and applications of fluorescence spectroscopy in this comprehensive 51-minute webinar presented by Aasmund Rinnan. Delve into the electromagnetic spectrum, molecular spectroscopy, and the fundamental principles of fluorescence. Learn about fluorophores, factors affecting fluorescence signals, and various measurement techniques including polarization and time-decay. Discover the advantages and limitations of this highly sensitive method capable of measuring compounds at ppb levels. Examine practical examples such as tryptophan fluorescence and the effects of environment, concentration, and quenching on fluorescence signals. Gain insights into advanced topics like the Second Order Advantage and the comparison between PLS and PARAFAC methods. Understand the applications of fluorescence spectroscopy in analyzing proteins and salt solutions, equipping yourself with valuable knowledge for research and analytical purposes.
Overview
Syllabus
Intro
Electromagnetic spectrum
What happens? Example: ketone
Molecular spectroscopy
Principles of spectroscopy
Principles of fluorescence
Tryptophan fluorescence
Fluorescence spectroscopy
Internal relaxation
Fluorescence dictionary - Part 11
Varian Eclipse
Xenon flash lamp
Instrumentation - PMT detector
Fluorophores - Molecular structure
Flourophores
Factors affecting the fluorescence signal
Concentration - Ideal conditions
Inner filter effect
Problem with the correction
Environment - Solvent
Environment - Temperature
Environment - Denaturant
Dynamic quenching
Static quenching
Non-radiative energy transfer
Scatter
Ways to measure fluorescence - Polarization
Ways to measure fluorescence - Time-decay
Fluorescence summary
Why fluorescence?
Options of measuring fluorescence
Second Order Advantage - PLS VS. PARAFAC
Proteins and salt solutions
Taught by
Chemometrics & Machine Learning in Copenhagen
