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NPTEL

Basics of Fluroscence Spectroscopy

NPTEL via Swayam

Overview

ABOUT THE COURSE:Fluorescence refers to light emission by substance after the absorption of electromagnetic radiation. Due to its environmental sensitivity and time resolved capability, fluorescence spectroscopy is now been widely applied to chemistry, biochemistry, biophysics, material science, forensic science, medicinal science, etc. Although fluorescence spectroscopy is used in many disciplines, this particular course is intended for the individuals willing to receive an in-depth introduction to the principles of fluorescence spectroscopy and its applications to chemistry and biology.PREREQUISITES:BS/B.Sc., Knowledge of basic quantum mechanicsINDUSTRY SUPPORT:Systronics India Ltd., B/116-129, 1st floor, Supath-II Complex, Nr.Juna Wadaj Bus Terminus, Ashram Road, Ahmedabad - 380013; Premier Colorscan Instruments Pvt. Ltd.

Syllabus

Week 1: Introduction to FluorescenceWeek 2: Instrumentation for Fluorescence Spectroscopy
Week 3: Time-Domain Lifetime Measurements
Week 4: Solvent and Environmental Effects
Week 5: Fluorescence Quenching
Week 6: Fluorescence Anisotropy
Week 7: Energy Transfer
Week 8: Single-Molecule Fluorescence

Taught by

Prof. Pratik Sen

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