Explore a 37-minute webinar on enhancing microbial detection sensitivity through unbiased human host DNA and RNA depletion. Learn from Dr. Zenas George, Senior Scientist at Jumpcode Genomics, as he presents cutting-edge research on characterizing the human microbiome. Discover how CRISPRclean technology leverages CRISPR-Cas9 to remove human nucleic acid fragments from microbiome samples, overcoming host contamination and maximizing microbial genome coverage. Gain insights into the experimental results, including increased species detection in clinical samples and improved depth of coverage for SARS-CoV-2. Understand the benefits of this innovative approach for achieving greater taxonomic resolution in whole metagenome sequencing studies.
Boost Microbial Detection Sensitivity with Unbiased Human Host DNA and RNA Depletion
Overview
Syllabus
Intro
Learning objectives
The vast majority of molecules sequenced are uninformative
The power of Jumpcode: Shift sequencing power to biologically relevant content
Applications and CRISPRclean product portfolio
Human microbiome samples
1-day workflow from DNA to ready-for-library-prep
Experimental Results
Unbiased human DNA depletion in contrived samples
Takeaways from Initial Comparison of Host Depletion Methods
Jumpcode has higher species counts correlation between untreated and depleted samples
3.7-fold boost in microbial reads in clinical samples (vaginal)
Detect more bacterial species in clinical samples (vaginal)
Detect more bacterial species in clinical samples (saliva)
Benefits Summary
Depletion of targeted human and Zymo Community Standard RNA, Increased depth of coverage for SARS-Cov-2
SARS-CoV-2 Depth of Coverage
Taught by
Labroots
