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Boost Microbial Detection Sensitivity with Unbiased Human Host DNA and RNA Depletion
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- 1 Intro
- 2 Learning objectives
- 3 The vast majority of molecules sequenced are uninformative
- 4 The power of Jumpcode: Shift sequencing power to biologically relevant content
- 5 Applications and CRISPRclean product portfolio
- 6 Human microbiome samples
- 7 1-day workflow from DNA to ready-for-library-prep
- 8 Experimental Results
- 9 Unbiased human DNA depletion in contrived samples
- 10 Takeaways from Initial Comparison of Host Depletion Methods
- 11 Jumpcode has higher species counts correlation between untreated and depleted samples
- 12 3.7-fold boost in microbial reads in clinical samples (vaginal)
- 13 Detect more bacterial species in clinical samples (vaginal)
- 14 Detect more bacterial species in clinical samples (saliva)
- 15 Benefits Summary
- 16 Depletion of targeted human and Zymo Community Standard RNA, Increased depth of coverage for SARS-Cov-2
- 17 SARS-CoV-2 Depth of Coverage
