Analytical Ultracentrifugation Capabilities for Gene Therapy Product Analysis

Join a 56-minute webinar led by Dr. Alexander Bepperling, a biochemist and biotechnology expert currently heading the Sandoz Biophysical Characterization lab, to explore advanced analytical ultracentrifugation (AUC) techniques for gene therapy product analysis. Discover how AUC provides multiple orthogonal assays for characterizing AAVs and LNPs, including determination of gene copy numbers, particle distribution, capsid titer, and particle density. Learn about the evolution of AUC applications, from traditional protein aggregation quantification to modern multi-dimensional analysis capabilities enabled by powerful computing. Explore various AUC methodologies including band SV-AUC, CsCl SE-AUC, boundary SV-AUC, and MWL-SV-AUC experiments, with special focus on determining mRNA copy numbers through D2O-concentration experiments. Master the industrial applications of AUC for characterizing gene transfer systems, understand the precision of modern AUC technology combined with state-of-the-art data analysis tools, and gain comprehensive knowledge of AUC's diverse analytical capabilities in gene therapy product development.