Characterization of Lipid Nanoparticles via Density Matching Analytical Ultracentrifugation

Join a 41-minute webinar exploring advanced analytical ultracentrifugation (AUC) methods for characterizing lipid nanoparticles (LNPs) loaded with nucleic acids. Learn from Dr. Amy Henrickson, an expert in biophysics and AUC, as she demonstrates innovative techniques using multiwavelength and D2O density matching to overcome size heterogeneity challenges in LNP analysis. Discover how to measure sedimentation and diffusion coefficient distributions of LNPs and their components, while understanding how density matching methods enable determination of partial specific volume distributions. Master the ability to differentiate between empty LNPs and various RNA loading states based on density measurements, as the significant density differences between RNA and lipids create proportional relationships to nanoparticle loading. Gain practical insights into applying these sophisticated analytical strategies beyond gene therapy drugs, including characterization of complex biopolymer interactions in biomedical research.