ABOUT THE COURSE:This course is designed for UG & PG students, research scholars and young scientists to introduce them to basics and applications of genome editing and engineering. The course starts with understanding of basic organization and structure of genome. It gives a brief overview on different DNA strand breaks and their repair mechanism. It introduces learners to theoretical basics of genetic engineering and discusses it’s limitations in tackling genetic problems of animals and plants. The key concepts of Genome editing are discussed thoroughly with emphasis on the major genome editing tools ZFN, TALEN and CRISP/Cas9. It discusses the biochemical basis of development of the genome editing tools, their design and their applications in various genetic conditions. It also discusses the scope and prospect of using these technologies in solving major genetic disease of human. The learners will also be acquainted with the ethical concerns associated with applications of genome editing and engineering in germline.INTENDED AUDIENCE: UG/PG/PhD/Scientist in industryPREREQUISITES:10+2 with Biology and Chemistry
Overview
Syllabus
Week 1:Introduction to genetics and genetic engineering
Week 2:Breakage and Repair Of Genomic DNA
Week 3:Recombination
Week 4:Targeted genetic modification
Week 5:Zinc Finger Nuclease (ZFN) Technology
Week 6:Transcription activator-like effector nuclease (TALEN) Technology
Week 7:Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology
Week 8:Applications of genome editing in treating human diseases
Week 9:Genome engineered Disease modeling
Week 10:Engineered immune cells for cancer therapy
Week 11:Personalized therapy; Challenges: safety and specificity
Week 12:Ethical concerns: Germ line gene editing
Taught by
Prof. Utpal Bora
