Overview

Due to rapidly evolving technological platforms in biology, there is a need to keep pace with latest developments in field to explore their versatile applications. Interactions resulting from protein-protein, protein-peptide, protein-RNA, protein-DNA or protein- small molecule have immense application in life-sciences and translational biology. Through this course, we aim to provide an interface between distinguished scientists involved in advanced interactomics research, industrial partners, faculties and students. This course would feature an intensive lecture series followed by some demonstrations designed to provide the much needed training required to explore the endless possibilities in interactomics research using genomics and proteomics approach, that can be useful for a researcher at any stage.This course of applications of interactomics using genomics and proteomics technologies focuses on two powerful platforms: Protein microarrays and Label-free biosensors and Next-Generation Sequencing (NGS) Technology.The students/participants will gain exposure to the above mentioned technologies and its applications from some of the experts in the field, from both- academia and industry perspective.

INTENDED AUDIENCE: Biology or biotechnology background students having interest in latest technologies, (BE/B.Tech) Biotechnology Students, B.Sc. students science background but course is open to all.

Syllabus

Week 1  : Introduction to Interactomics and Protein Arrays ,Basics and Applications of Reverse Phase Protein Arrays-I,Basics and Applications of Reverse Phase Protein Arrays-IIBasics and Applications of Reverse Phase Protein Arrays-III,Weekly Perspective-I
Week 2  : NAPPA Technology and Protein Arrays , NAPPA Technology and Protein Arrays-II, Using functional proteomics to identify biomarkers and therapeutic targets , Using functional proteomics to identify biomarkers and therapeutic targets-II, Weekly Perspective-II
Week 3  : NAPPA Arrays: Workflow, NAPPA and its applications in study of antibody immune response in disease and in drug screening-I, NAPPA and its applications in study of antibody immune response in disease and in drug screening-II, NAPPA and its applications in study of antibody immune response in disease and in drug screening-III, Weekly Perspective -III
Week 4  : Biomarkers: Harnessing the immune system for early detection of disease-I, Biomarkers: Harnessing the immune system for early detection of disease-II, Biomarkers: Harnessing the immune system for early detection of disease-III, Multi-variate data analysis to identify potential leads from proteomic datasets, Multi-variate data analysis to identify potential leads from proteomic datasets
Week 5  : The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-I , The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-II, Antibody signatures defined by high-content peptide microarray analysis
Week 6  : An overview of label-free technologies-I, An overview of label-free technologies-II, Mass Spectrometry coupled Interactomics-I, Mass Spectrometry coupled Interactomics-II, Biomolecular interactions using Bio-Layer Interferometry (BLI)
Week 7  : Surface Plasmon Resonance- Principles and Assays-I, Surface Plasmon Resonance- Principles and Assays-II, SPR Application, Biomolecular interaction analytics using MicroScale Thermophoresis , NGS target enrichment workflow for exomes, targeted panels and beyond
Week 8  : Next-Gen Sequencing Technology-I, Next-Gen Sequencing Technology-II, Next-Gen Sequencing Technology-III, Next-Gen Sequencing Technology-IV , Summary of high-throughput technologies to study DNA and protein interactions