RISCy Business - Development of a RNAi Design and Off-Target Prediction Software

RISCy Business - Development of a RNAi Design and Off-Target Prediction Software

EuroPython Conference via YouTube Direct link

Results 100 bp window constructs

12 of 14

12 of 14

Results 100 bp window constructs

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RISCy Business - Development of a RNAi Design and Off-Target Prediction Software

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  1. 1 Intro
  2. 2 Finding genes in a haystack
  3. 3 How RNAi works
  4. 4 Inbuilt RNAi issues
  5. 5 Possible solution
  6. 6 What is an off-target?
  7. 7 Experiment setup
  8. 8 Results for silencing efficiency
  9. 9 Which siRNAs are efficient?
  10. 10 Strand selection
  11. 11 Target site accessibility calculation
  12. 12 Results 100 bp window constructs
  13. 13 si-Fi Features
  14. 14 si-Fi example

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