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Inbuilt RNAi issues
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Classroom Contents
RISCy Business - Development of a RNAi Design and Off-Target Prediction Software
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- 1 Intro
- 2 Finding genes in a haystack
- 3 How RNAi works
- 4 Inbuilt RNAi issues
- 5 Possible solution
- 6 What is an off-target?
- 7 Experiment setup
- 8 Results for silencing efficiency
- 9 Which siRNAs are efficient?
- 10 Strand selection
- 11 Target site accessibility calculation
- 12 Results 100 bp window constructs
- 13 si-Fi Features
- 14 si-Fi example
