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EPFL Regularized reconstruction scheme for SPA
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Cryo-Electron Microscopy and Single Particle Analysis - Webinar 3
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- 1 Intro
- 2 EPFL Outline
- 3 EPFL Cryo-electron microscopy: imaging biosamples at high-resolution in their native state
- 4 EPFL The fundamental principles of SPA
- 5 EPFL The transmission of straight electron rays is modeled with the X-ray transform
- 6 EPFL The 3D object is represented in a shift-invariant ba
- 7 EPFL Tomographic reconstruction in SPA is a major technical challenge
- 8 EPFL Current reconstruction paradigms achieve high-res through iterative-refinement procedures
- 9 EPFL Regularized reconstruction scheme for SPA
- 10 EPFL The joint scheme appropriately refines the structure and the orientations
- 11 EPFL The scheme compares favorably with
- 12 EPFL Classical GANs estimate data distributions through an adversarial learning scheme
- 13 EPFL CryoGAN relies on a mathematical framework that provides strong recovery guarantees
- 14 EPFL A closer look on the min-max problem
- 15 EPFL The CryoGAN algorithm
- 16 EPFL Synthetic Experiment (beta-gal)
- 17 EPFL Discriminator architecture
- 18 EPFL Results (Structure)
- 19 SPRL Reconstruction (Structure)
- 20 EPFL Structural heterogeneity: the key challenge ahead
- 21 EPFL Reconstruction of a continuum of conformations
- 22 EPFL Reconstruction for a discrete set of conformations
- 23 EPFL Conclusion
