Cryo-Electron Microscopy and Single Particle Analysis - Webinar 3

Cryo-Electron Microscopy and Single Particle Analysis - Webinar 3

IEEE Signal Processing Society via YouTube Direct link

Intro

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1 of 23

Intro

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Cryo-Electron Microscopy and Single Particle Analysis - Webinar 3

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  1. 1 Intro
  2. 2 EPFL Outline
  3. 3 EPFL Cryo-electron microscopy: imaging biosamples at high-resolution in their native state
  4. 4 EPFL The fundamental principles of SPA
  5. 5 EPFL The transmission of straight electron rays is modeled with the X-ray transform
  6. 6 EPFL The 3D object is represented in a shift-invariant ba
  7. 7 EPFL Tomographic reconstruction in SPA is a major technical challenge
  8. 8 EPFL Current reconstruction paradigms achieve high-res through iterative-refinement procedures
  9. 9 EPFL Regularized reconstruction scheme for SPA
  10. 10 EPFL The joint scheme appropriately refines the structure and the orientations
  11. 11 EPFL The scheme compares favorably with
  12. 12 EPFL Classical GANs estimate data distributions through an adversarial learning scheme
  13. 13 EPFL CryoGAN relies on a mathematical framework that provides strong recovery guarantees
  14. 14 EPFL A closer look on the min-max problem
  15. 15 EPFL The CryoGAN algorithm
  16. 16 EPFL Synthetic Experiment (beta-gal)
  17. 17 EPFL Discriminator architecture
  18. 18 EPFL Results (Structure)
  19. 19 SPRL Reconstruction (Structure)
  20. 20 EPFL Structural heterogeneity: the key challenge ahead
  21. 21 EPFL Reconstruction of a continuum of conformations
  22. 22 EPFL Reconstruction for a discrete set of conformations
  23. 23 EPFL Conclusion

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