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Single-Molecule Proteomics Using Protein Identification by Short-epitope Mapping (PrISM)
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- 1 Introduction
- 2 Background
- 3 Challenges
- 4 Key Criteria
- 5 Nautilus Approach
- 6 Sample Preparation
- 7 Sample Prep Workflow
- 8 Identification
- 9 Workflow
- 10 Building Multiaffinity Probes
- 11 Is there a magical perfect set of targets
- 12 Data from a larger experiment
- 13 Computational analyses
- 14 Targeted proteomics
- 15 Why an intact protein approach
- 16 Targeted protein analysis
- 17 Tau protein analysis
- 18 Key aspirational goals
- 19 Single Molecule Proteomics First Access Challenge
- 20 Live QA
- 21 Factors for false identification
- 22 Databases used for protein identification
- 23 Limitations to sample types
- 24 Data analysis time
- 25 How to remove high affinity antibodies
- 26 Major applications of the platform
