Single-Molecule Proteomics Using Protein Identification by Short-epitope Mapping (PrISM)

Single-Molecule Proteomics Using Protein Identification by Short-epitope Mapping (PrISM)

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Background

2 of 26

2 of 26

Background

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Classroom Contents

Single-Molecule Proteomics Using Protein Identification by Short-epitope Mapping (PrISM)

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  1. 1 Introduction
  2. 2 Background
  3. 3 Challenges
  4. 4 Key Criteria
  5. 5 Nautilus Approach
  6. 6 Sample Preparation
  7. 7 Sample Prep Workflow
  8. 8 Identification
  9. 9 Workflow
  10. 10 Building Multiaffinity Probes
  11. 11 Is there a magical perfect set of targets
  12. 12 Data from a larger experiment
  13. 13 Computational analyses
  14. 14 Targeted proteomics
  15. 15 Why an intact protein approach
  16. 16 Targeted protein analysis
  17. 17 Tau protein analysis
  18. 18 Key aspirational goals
  19. 19 Single Molecule Proteomics First Access Challenge
  20. 20 Live QA
  21. 21 Factors for false identification
  22. 22 Databases used for protein identification
  23. 23 Limitations to sample types
  24. 24 Data analysis time
  25. 25 How to remove high affinity antibodies
  26. 26 Major applications of the platform

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