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FastQ to BAM - Align reads to the reference genome with STAR - Step 5
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Classroom Contents
Sequencing Tutorial
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- 1 Downloading sequencing data on windows - SRA Tool kit - Step 1
- 2 Converting SRA into FASTQ file - SRA Tool kit - Step 2
- 3 FASTQ quality check (FastQC) - Step 3
- 4 Trimming of FastQ file (Trimmomatic) - Step 4
- 5 FastQ to BAM - Align reads to the reference genome with STAR - Step 5
- 6 Indexing BAM file using samtools - Step 6
- 7 Caculate gene expression with FeatureCounts - Step 7
- 8 Downloading sequencing data on ubuntu/linux - SRA toolkit
