Sequencing Tutorial

Sequencing Tutorial

Farhan Haq via YouTube Direct link

FASTQ quality check (FastQC) - Step 3

3 of 8

3 of 8

FASTQ quality check (FastQC) - Step 3

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Sequencing Tutorial

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  1. 1 Downloading sequencing data on windows - SRA Tool kit - Step 1
  2. 2 Converting SRA into FASTQ file - SRA Tool kit - Step 2
  3. 3 FASTQ quality check (FastQC) - Step 3
  4. 4 Trimming of FastQ file (Trimmomatic) - Step 4
  5. 5 FastQ to BAM - Align reads to the reference genome with STAR - Step 5
  6. 6 Indexing BAM file using samtools - Step 6
  7. 7 Caculate gene expression with FeatureCounts - Step 7
  8. 8 Downloading sequencing data on ubuntu/linux - SRA toolkit

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