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Measuring NE enrichment using nuclear localization index NLI
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Classroom Contents
A Nuclear Localization Signal Targets Tail-Anchored Membrane Proteins to the Inner Nuclear Envelope in Plants
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- 1 Intro
- 2 Soluble proteins are actively imported into the nucleus, through binding of the NLS
- 3 How do proteins traffic to the Inner Nuclear Membrane?
- 4 Models for membrane protein transport to the INM
- 5 Transport-Factor Mediated Trafficking of inner nuclear membrane proteins
- 6 Experimental Questions
- 7 Targeting a tail anchored ER protein to the nuclear envelope
- 8 Measuring NE enrichment using nuclear localization index NLI
- 9 Addition of an NLS to an chimeric membrane protein results in NE enrichment
- 10 Defining Rules for NE Targeting
- 11 NLS fused chimeric membrane proteins access
- 12 Working model of NLS-mediated INM protein trafficking
- 13 Addition of a monopartite NLS is sufficient to enrich an artificial membrane protein at the NE
