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Detecting Viruses with CRISPR: Applications and Technologies
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- 1 Intro
- 2 Where do we want to detect viruses?
- 3 CRISPR allows bacteria to defend themselves against phages
- 4 Cas13 is the RNA-targeting equivalent of Cas9
- 5 Cas13 has similar potential to Cas9 but for RNA
- 6 Using Cas13 to detect nucleic acids
- 7 SHERLOCK uses Cas13 for sensitive nucleic acid detection
- 8 SHERLOCK can work at the point of care
- 9 SHINE V1 (Streamlined Highlighting of Infections to Navigate Epidemics)
- 10 SHINE v2: towards at-home COVID-19 testing
- 11 SHINE v2 outperforms rapid antigen tests
- 12 SHINE v2 can be incubated using body heat
- 13 Can we scale up SHERLOCK?
- 14 CARMEN - SHERLOCK at massive multiplex
- 15 Microfluidic CARMEN (mCARMEN)
- 16 Developing a CARMEN respiratory virus panel (RVP)
- 17 Streamlining the workflow of CARMEN-RVP
- 18 CARMEN-RVP has exquisite analytical sensitivity
- 19 The CARMEN Variant Identification Panel (VIP)
- 20 SARS-CoV-2 lineage identification with the VIP
- 21 Omicron produces a unique signature on the VIP
- 22 Tracking the spread of Omicron in Massachussetts
- 23 Acknowledgements
- 24 We are recruiting postdocs!
