Accessing the Nanoscale with MINFLUX Light Microscopy

Explore the groundbreaking MINFLUX concept for localizing photon emitters in space through this illuminating conference talk. Delve into the innovative technique that minimizes fluorescence photons required for high localization precision, achieving 22 times fewer photons compared to popular centroid localization methods. Discover how MINFLUX superresolution microscopy attains ~1-nanometer precision, resolving molecules merely 6 nanometers apart. Learn about the remarkable advancements in single fluorescent protein tracking, which increase temporal resolution and localizations per trace by a factor of 100, as demonstrated with diffusing 30S ribosomal subunits in living Escherichia coli. Gain insights into the potential of this localization modality to revolutionize observations of macromolecule dynamics, distribution, and structure in living cells and beyond, as conceptual limits have yet to be reached.