Overview
Syllabus
Intro
we can select a series of enzymes from a catalog that will produce some desired industrial compound
petroleum/crude oil
methyl methacrylate (MMA)
advantages of PMMA
production of PMMA from crude oil is not sustainable
oil in the ground will eventually run out
Project Lead: Aaron Korkegian
a-methylene-y-butyrolactone (MBL)
fermentation host (yeast, bacteria, etc.)
enzyme database + efficient algorithm
natural substrate
the methyl may clash in the active site
Proposed Pathway
Generating the Enzymes
Testing the Enzymes
Optimizing the Enzymes
Testing the Optimized Enzymes
Confirmed Pathway
we can modify the ribosomal binding site sequences for any of the enzymes
any such reaction can be run in precisely the same large vat with the same material
PROFESSOR DAVE EXPLAINS
Taught by
Professor Dave Explains