How CRISPR Is Revolutionizing Cancer Research

Introduction.
Cancer is a huge global health problem.
Some cancer types are well treated while others remain refractory to current strategies.
Cancer is fundamentally a genetic disease.
Tumors have multiple layers of genomic diversity.
CRISPR/Cas9 and many other genome editing systems exist naturally in bacterial and archaea.
Expression of Case and an SERNA in mammalian cells can inactivate a gene of interest.
Two major areas where CRISPR/Cas9-mediated genome editing has accelerated our understanding of cancer.
Libraries of SgRNAs can be used to inactivate many genes of interest in parallel.
Outline of experiment to discover pairs of genes that when inactivated kill cancer cells.
Use the information from the paired CRISPR screen to find effective drug combinations.
Two major areas where CRISPR/Cass-mediated genome editing has accelerated our understanding of cancer.
Use of CRISPR/Cas9 to understand the function of single genes of interest.
Identification of candidate tumor suppressor genes from human lung cancer sequencing data.
Multiplexed genome editing and high throughput barcode sequencing to assess tumor suppression.
Multiplexed tumor suppressor targeting leads to rapid tumor growth.
Multiplexed analysis of gene function in lung cancer in vivo helps understand tumor suppression.
Cancers often have alterations in multiple tumor suppressor genes.
Inactivation of different combinations of genes has dramatically different effects on cancer growth.
Complex functional taxonomy of tumor suppression in lung cancer NORMAL.
Personalized medicine is based on understanding how different tumors will respond to different therapies.
Multiplexed genome editing to uncover how genes impact drug responses.
Surprisingly complex map of genotype-specific therapeutic responses.
What aspects of carcinogenesis remain less well understood?.