Explore single-molecule proteomics through Protein Identification by Short-epitope Mapping (PrISM) in this 51-minute webinar presented by Dr. Parag Mallick. Delve into the revolutionary approach that enables comprehensive protein analysis with increased sensitivity, reproducibility, and accessibility. Learn about the innovative method of immobilizing intact proteins, iteratively probing them with multi-affinity probes, and applying machine learning to convert binding patterns into protein identification and quantification. Discover how PrISM utilizes non-traditional affinity reagents to bind short epitopes in multiple proteins, potentially identifying over 95% of the human proteome with just 300 probes. Examine the experimental setup, including DNA nanoparticle conjugation and high-density patterned flow cells. Gain insights into the challenges, key criteria, and workflow of this groundbreaking technique. Explore targeted proteomics, intact protein analysis, and the potential applications of this platform in biological research and healthcare. Engage with the content through live Q&A sessions covering topics such as false identification factors, databases used, sample type limitations, and data analysis time.
Single-Molecule Proteomics Using Protein Identification by Short-epitope Mapping (PrISM)
Overview
Syllabus
Introduction
Background
Challenges
Key Criteria
Nautilus Approach
Sample Preparation
Sample Prep Workflow
Identification
Workflow
Building Multiaffinity Probes
Is there a magical perfect set of targets
Data from a larger experiment
Computational analyses
Targeted proteomics
Why an intact protein approach
Targeted protein analysis
Tau protein analysis
Key aspirational goals
Single Molecule Proteomics First Access Challenge
Live QA
Factors for false identification
Databases used for protein identification
Limitations to sample types
Data analysis time
How to remove high affinity antibodies
Major applications of the platform
Taught by
Labroots
