Dive into a comprehensive 10-hour course on bioinformatics, covering both theoretical concepts and practical applications. Learn about fundamental topics such as sequence alignment, FASTA and BLAST algorithms, global and local alignments, and secondary structure prediction. Explore various bioinformatics tools and databases, including NCBI BLAST, ClustalW, and Protein Data Bank. Gain hands-on experience with practical sessions on database searching, multiple sequence alignment, phylogenetic tree construction, and protein structure analysis. Master essential skills like primer design, restriction mapping, and identifying conserved domains in protein sequences. By the end of this course, develop a strong foundation in bioinformatics techniques and their applications in biological research.
Overview
Syllabus
Bioinformatics part 1 What is Bioinformatics.
Bioinformatics part 2 Databases (protein and nucleotide).
Bioinformatics part 3 Sequence alignment introduction.
Bioinformatics part 4 Introduction to FASTA and BLAST.
Bioinformatics part 5 FASTA algorithm.
Bioinformatics part 6 BLAST algorithm.
Bioinformatics part 7 How to perform Global alignment 1.
Bioinformatics part 8 How to perform Global alignment 2.
Bioinformatics part 9 how to align sequences using trace back method.
Bioinformatics part 10 How to perform local alignment.
Bioinformatics part 11 Sequence motifs and PROSITE notations.
Bioinformatics part 12 secondary structure prediction using Chou Fasman method.
Bioinformatics part 13 how to calculate the propensity value.
Bioinformatics par 14: Dot plot.
Bioinformatics par 15 pairwise alignment.
Bioinformatics part 16 evolution of bioinformatics tools.
Bioinformatics part 17 homolog analog xenolog.
Bioinformatics practical introduction.
Bioinformatics Practical 1 database searching and retrival of sequence.
Bioinformatics practical 2 how to run NCBI BLAST.
Bioinformatics prac 3 Conserved domain search.
Bioinformatics practical 4 multiple sequence alignment using ClustalW.
Bioinformatics practical 5 Phylogenetic tree construction.
Bioinformatics practical 6 Phylogenetic tree construction using MABL.
Bioinformatics practical 7 Secondary structure prediction of proteins using SIB.
Bioinformatics practical 7 secondary structure prediction 2.
Bioinformatics practical 8 retrieving sequence fom Protein data bank.
Bioinformatics practical 21 how to find transmembrane region in protein sequence.
Bioinformatics practical 24 how to use PROSITESCAN.
Bioinformatics practical 23 motif scan tool to identify known domains in protein sequence.
Bioinformatics practical 22 how to search for known domains in protein sequence.
Bioinformatics course 20 how to know the physicochemical properties of a protein.
Bioinformatics practical 19 how to make a restriction map.
Bioinformatics practical 18 how to predict translation product of a gene sequence?.
Bioinformatics lecture 17 primer dimer check (bioinformatics practical).
Bioinformatics lecture 16 primer design.
Bioinformatics lecture 15 how to screen vector contamination using vecscreen.
Bioinformatics lecture 14 how to calculate % GC content and molecular weight of a gene.
Bioinformatics lecture 12 ORF finder in NCBI (practical bioinformatics).
Bioinformatics lecture 11 gene centric databases (practical bioinformatics).
Bioinformatics lecture 10 whole genome database (practical bioinformatics).
Bioinformatics lecture 9 working with eukaryotic DNA (practical bioinformatics).
Taught by
Shomu"s Biology
