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Explore a practical approach to implementing cost-effective and clinically relevant work-ups of respiratory cultures in this 33-minute lecture by Dr. Yvette S. McCarter, professor at the University of Florida College of Medicine–Jacksonville. Learn about the pathogenesis of pneumonia, the utility of Gram stain, screening criteria, mixed flora criteria, and "Q" systems advantages. Examine real-world examples using sputum and tracheal aspirate samples. Gain insights into integrating cost-effectiveness and clinical relevance into daily microbiology practice, addressing challenges faced by clinical microbiologists. This American Society for Microbiology virtual lecture, presented by a fellow of the American Academy of Microbiology, offers valuable knowledge for microbiologists seeking to enhance their respiratory culture work-up techniques.